Equipment and Access

Zeiss LSM880 Confocal Microscope

System location: HBTM 9th floor 09012C
To schedule a training session or pilot on the system, please contact Lai Ding ( lding@bwh.harvard.edu) or Teri Zgoda (tzgoda@bwh.harvard.edu).

Reservation is through the Partners Core Management System (PCMS). Users need to register through the PCMS website (see below), then request training.

https://researchcores.partners.org/nts/about

 

Available Techniques: Confocal, Super-Resolution, Live-cell Imaging, Brightfield, DIC

Objectives: 10x, 20x, 40x (dry), 40x (water), 40x, 63x (oil)

 

This Zeiss flagship confocal system offers high sensitivity, enhanced resolution in x, y and z, and high image-acquisition speed in one system. The patent Airyscan feature extends the system’s ability on Sensitivity, Resolution and Speed. Users achieve a 1.7× higher resolution in all spatial dimensions, 140 nm laterally and 400 nm axially. The improved sensitivity leads to better image quality and increased speed. The whole imaging process is possible with standard sample preparation and labeling protocols. The Zeiss LSM880 lets the user take full advantage of large fields of view and the highest speed of any linear scanning confocal microscope at an unsurpassed image quality.

Zeiss LSM710 Confocal Microscope

System location: HBTM 10th floor 10006B
To schedule a training session or pilot on the system, please contact Lai Ding ( lding@bwh.harvard.edu) or Teri Zgoda (tzgoda@bwh.harvard.edu).

Reservation is through the Partners Core Management System (PCMS). Users need to register through the PCMS website (see below), then request training.

https://researchcores.partners.org/nts/about

 

Available Techniques: Confocal, Brightfield, DIC

Objectives: 10x, 20x, 40x (dry), 40x (water), 40x, 63x, 100x (oil)

 

This LSM710 confocal system is equipped with multiple laser lines ranging from 405nm to 633nm, and with a 32-channel Quasar spectral detector.  The system uses a Zeiss AXIO Observer Z1 inverted microscope stand with transmitted (HAL), UV (HBO) illumination sources. It can collect transmitted light images (bright field and DIC) as well as conventional and confocal fluorescence images.

Leica DMi8 Widefield Microscope


System location:
 HBTM 10th floor 10006B
To schedule a training session or pilot on the system, please contact Lai Ding ( lding@bwh.harvard.edu) or Teri Zgoda (tzgoda@bwh.harvard.edu).

Reservation is through the Partners Core Management System (PCMS). Users need to register through the PCMS website (see below), then request training.

https://researchcores.partners.org/nts/about

 

Available Techniques: Fluorescent, Brightfield, DIC, Phase Contrast, Histology Color Imaging, Live-Cell Imaging, Whole-Slide Scan

Objectives: 2.5x, 5x, 10x, 20x, 40x (dry), 40x (water), 40x, 63x, 100x (oil)

 

The Leica DMi8 is a versatile widefield microscope system suitable for fluorescence, brightfield, contrast method (phase contrast, DIC, polarized), histology color imaging and live-cell imaging (with OKO lab on-stage incubator). The system can be used to image multiple sample types including slides, Petri dishes, multi-well plates, and small flasks. It is also capable of large tile stitching and can be operated as a whole slide scanning instrument.

 

GE IN Cell Analyzer 2200

System location: HBTM 7th floor 07006D
To schedule a training session or pilot on the system, please contact Lai Ding ( lding@bwh.harvard.edu) or Teri Zgoda (tzgoda@bwh.harvard.edu).

Reservation is through the Partners Core Management System (PCMS). Users need to register through the PCMS website (see below), then request training.

https://researchcores.partners.org/nts/about

 

Available Techniques: Fluorescent, Brightfield, High-Content Imaging, Live-Cell Imaging, Whole-Slide Scan

Objectives: 10x, 20x, 40x (dry)

 

This super-fast, sensitive, and flexible widefield cell imaging system is suitable for a broad range of high-content assays (HCA), including compound screening, predictive toxicology, protein localization and trafficking, functional studies, and stem cell analysis.

 

The IN Cell Analyzer 2200 incorporates intuitive and easy-to-use tools that simplify the imaging process for different sample types and enable a wide range of applications. It makes both simple and complex high-throughput, high-content assays an everyday reality.

 

Highlights of the system configuration:

  • 5MP high-performance large field-of-view sCMOS camera.
  • Seven wavelength solid-state light source covers fluorescence dyes ranging from blue (DAPI) to infrared (Cy5) spectrum.
  • Environmental chamber for temperature and humidified CO2content control.
  • FRET imaging module (CFP-YFP pair).
  • Transmitted light imaging module.
  • Fast and robust hardware autofocus + software focus.
  • Deconvolution 3-D image restoration.
  • GE image analysis software fleet including “IN Carta”, “Investigator” and “Developer”.

Agilent Seahorse

System location: HBTM 7th floor 07006

To schedule a training session or pilot on the system, please contact Lai Ding (lding@bwh.harvard.edu) or Teri Zgoda (tzgoda@bwh.harvard.edu). 

Reservation and training requests are through the PCMS website.

https://researchcores.partners.org/nts/about

 

The Agilent Seahorse XFe96 Analyzer measures oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a 96-well plate, interrogating key cellular functions such as mitochondrial respiration and glycolysis. XF Analyzers perform compound addition and mixing, label-free analytical detection, and automatic measurement of OCR and ECAR in real time.

There is a small incubator adjacent to the instrument, for storing cells prior to testing.

Mesoscope

System location: HBTM 11th floor 11128

The mesoscope is not yet incorporated into the Partners Core Management System (PCMS), although we plan to do this in future.  Until then, investigators interested in performing pilot studies on the system may contact Lai Ding ( lding@bwh.harvard.edu) or Teri Zgoda (tzgoda@bwh.harvard.edu) to discuss research interests and training requirements. 

 

The Thorlabs Mesoscope provides 2-photon imaging at subcellular resolution (~0.6 micron) over a very wide (~5mm) field of view, at near-video frame rates. It is designed for intravital imaging in small animals, including the ability to monitor neural activity across multiple brain regions in parallel.

Single Cell Genomics

The Neurotechnology Studio has partnered with the Division of Rheumatology, Immunology and Allergy (BWH Dept. of Medicine) and the Evergrande Center for Immunologic Diseases to establish a single cell genomics core facility that is available to members of the BWH neuroscience research community.   The core offers single-cell RNA sequencing (sc-RNAseq) based on droplet sequencing technology from 10X Genomics.  The standard service includes construction of bar-coded libraries, sequencing, and a standardized bioinformatics analysis based on 10X Genomics pipelines.  The core also offers individual consultations for users seeking help with custom analysis.

Location:  Hale Building for Transformative Medicine, 60 Fenwood Road, Room 6022C

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Staff:  Adam Chicoine (core manager); Kevin Wei, MD, Ph.D (scientific co-director)

Email: SingleCell@bwh.harvard.edu

FACS Analyzer

The Neurotechnology Studio has acquired a BD Biosciences FACSymphony analyzer, which is housed within the ARCND flow cytometry core, and is available to all members of the BWH neuroscience research community.  The FACSympony is the most powerful analytical FACS instrument on the market with 5 lasers, capable of sorting up to 29 fluorochromes.  The instrument is also equipped with a high-throughput system for running samples in 96-well plates.  A custom range of labeled antibodies is currently under development.

 

Location:   Hale Building for Transformative Medicine, 60 Fenwood Road, Room 9014

 

For more information contact Clare Baecher-Allen, Ph.D, senior core manager: CALLAN@RICS.BWH.HARVARD.EDU

Leica RT2125 Rotary Microtome


System location:
 HBTM 7th floor 07006
To schedule a training session or pilot on the system, please contact Lai Ding ( lding@bwh.harvard.edu) or Teri Zgoda (tzgoda@bwh.harvard.edu).

Reservation is through the Partners Core Management System (PCMS). Users need to register through the PCMS website (see below), then request training.

https://researchcores.partners.org/nts/about

 

The Leica RM2125 RTS manual microtome provides high-quality sections which range from 0.5mm to 60 mm thickness.

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