Equipment and Access

Zeiss LSM880 Confocal Microscope

System location: BTM 10th floor, Room 09012C
To schedule a training session or pilot on the system, please contact Lai Ding, PhD: lding@bwh.harvard.edu. 

To reserve specific time for equipment usage, see link below for calendar access. (Note: Only trained users may access schedule via link.)

https://www.brownbearsw.com/mc/NeuroTechnologyStudio_Imaging

 

This Zeiss flagship confocal system offers high sensitivity, enhanced resolution in x, y and z, and high image-acquisition speed in one system. The patent Airyscan feature extends the system’s ability on Sensitivity, Resolution and Speed. Users achieve a 1.7× higher resolution in all spatial dimensions, 140 nm laterally and 400 nm axially. The improved sensitivity leads to better image quality and increased speed. The whole imaging process is possible with standard sample preparation and labeling protocols. Zeiss LSM880 lets the user take full advantage of large fields of view and the highest speed of any linear scanning confocal microscope at an unsurpassed image quality.

GE IN CELL ANALYZER 2200

System location: BTM 10th floor, Room 10006D
To schedule a training session or to test equipment, please contact Lai Ding, PhD: lding@bwh.harvard.edu. 

To reserve specific time for equipment usage, see link below for calendar access. (Note: Only trained users may access schedule via link): 

 https://www.brownbearsw.com/mc/NeuroTechnologyStudio_Imaging

 

This super-fast, sensitive, and flexible widefield cell imaging system is suitable for a broad range of high-content assays (HCA), including compound screening, predictive toxicology, protein localization and trafficking, functional studies, and stem cell analysis.

 

IN Cell Analyzer 2200 incorporates intuitive and easy-to-use tools that simplify the imaging process for different sample types and enable a wide range of applications. It makes both simple and complex high-throughput, high-content assays an everyday reality.

 

Highlights of the system configuration:

  • 5MP high-performance large field-of-view sCMOS camera.
  • Seven wavelength solid-state light source covers fluorescence dyes ranging from blue (DAPI) to infrared (Cy5) spectrum.
  • Environmental chamber for temperature and humidified CO2 content control.
  • FRET imaging module (CFP-YFP pair).
  • Transmitted light imaging module.
  • Fast and robust hardware autofocus + software focus.
  • Wide range choices of objectives: 2X, 10X, 20X and 40X with automated correction collar.
  • Deconvolution 3-D image restoration.
  • GE image analysis software fleet including “IN Carta”, “Investigator” and “Developer”.

Leica RT2125 Rotary Microtome

System location: BTM 9th floor, Room 9006.10
To schedule a training session or to test equipment, please contact Lai Ding, PhD: lding@bwh.harvard.edu. 

 

Leica RM2125 RTS manual microtome provides high-quality sections which range from 0.5um to 60um thickness.

Zeiss LSM710 Confocal Microscope

This confocal system was acquired through a NIH Shared Instrument Grant (S10) in 2013 (PI: Tracy Young-Pearse).  Dr. Lai Ding of the NeuroTechnology Studio manages the system’s day-to-day operation, training and maintenance.

 

This LSM710 confocal system, equipped with multiple laser lines range from 405nm to 633nm and 32-channel Quasar spectral detector, is capable of continuous spectral detection with up to 10 dyes used simultaneous. With environmental chamber and define focus system, the system is ideal to perform live cell imaging project.

 

The system may be reserved by authorized users through the online calendar:

https://www.brownbearsw.com/cal/cndconfocal

 

For training or questions regarding this system, please contact Dr. Lai Ding: lding@bwh.harvard.edu

This confocal system was acquired through a NIH Shared Instrument Grant (S10) in 2013 (PI: Tracy Young-Pearse).  Dr. Lai Ding of the NeuroTechnology Studio manages the system’s day-to-day operation, training and maintenance.

 

This LSM710 confocal system, equipped with multiple laser lines range from 405nm to 633nm and 32-channel Quasar spectral detector, is capable of continuous spectral detection with up to 10 dyes used simultaneous. With environmental chamber and define focus system, the system is ideal to perform live cell imaging project.

 

The system may be reserved by authorized users through the online calendar:

https://www.brownbearsw.com/cal/cndconfocal

 

For training or questions regarding this system, please contact Dr. Lai Ding: lding@bwh.harvard.edu

Mesoscope

The Neurotechnology Studio has acquired a Thorlabs Mesoscope, which provides 2-photon imaging at subcellular resolution (~0.6 micron) over a very wide (~5mm) field of view, at near-video frame rates. It is designed for intravital imaging in small animals, including the ability to monitor neural activity across multiple brain regions in parallel.  The mesoscope was installed in May 2018 and is currently undergoing testing.  It expected to be available for use in summer 2018.  The system will also include a computer for short-term storage of the large datasets produced by this instrument. (Long-term storage will be the responsibility of individual users.)

 

System location: Hale Building for Transformative Medicine, 60 Fenwood Road, Room 11128

 

For more information contact Lai Ding, Ph.D (lding@bwh.harvard.edu) or  Stelios Smirnakis, MD PhD (smsmirnakis@bwh.harvard.edu).

 

SINGLE CELL GENOMICS

The Neurotechnology Studio has partnered with the Division of Rheumatology, Immunology and Allergy (BWH Dept. of Medicine) and the Evergrande Center for Immunologic Diseases to establish a single cell genomics core facility that is available to members of the BWH neuroscience research community.   The core offers single-cell RNA sequencing (sc-RNAseq) based on droplet sequencing technology from 10X Genomics.  The standard service includes construction of bar-coded libraries, sequencing, and a standardized bioinformatics analysis based on 10X Genomics pipelines.  The core also offers individual consultations for users seeking help with custom analysis.

Location:  Hale Building for Transformative Medicine, 60 Fenwood Road, Room 6022C

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Staff:  Adam Chicoine (core manager); Kevin Wei, MD, Ph.D (scientific co-director)

Email: SingleCell@bwh.harvard.edu

FACS Analyzer

The Neurotechnology Studio has acquired a BD Biosciences FACSymphony analyzer, which is housed within the ARCND flow cytometry core, and is available to all members of the BWH neuroscience research community.  The FACSympony is the most powerful analytical FACS instrument on the market with 5 lasers, capable of sorting up to 29 fluorochromes.  The instrument is also equipped with a high-throughput system for running samples in 96-well plates.  A custom range of labeled antibodies is currently under development.

 

Location:   Hale Building for Transformative Medicine, 60 Fenwood Road, Room 9014

 

For more information contact Clare Baecher-Allen, Ph.D, senior core manager: CALLAN@RICS.BWH.HARVARD.EDU

LEICA DMi8 WIDE FIELD MICROSCOPE

System location: BTM 10th floor, Room 10006B
To schedule a training session or to test equipment, please contact Lai Ding, PhD: lding@bwh.harvard.edu. 

 

To reserve specific time for equipment usage, see link below for calendar access. (Note: Only trained users may access schedule via link): 

 https://www.brownbearsw.com/mc/NeuroTechnologyStudio_Imaging

 

The Leica DMi8 system is a versatile widefield system suitable for fluorescence, brightfield, contrast method (phase contrast, DIC, polarized) and histology color imaging. The system can be used to image multiple sample types including slides, Petri dishes, multi-well plates, and small flasks. It is also capable of large tile stitching and can be operated as a whole slide scanning instrument.

 

 

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