Leica DMi8 Widefield Microscope


System location: HBTM 10th floor 10006B

To schedule a training session or pilot on the system, please contact Lai Ding ( lding@bwh.harvard.edu)

Reservation is through the Partners Core Management System (PCMS). Users need to register through the PCMS website (see below), then request training.



Available Techniques: Fluorescent, Brightfield, DIC, Phase Contrast, Histology Color Imaging, Live-Cell Imaging, Whole-Slide Scan

Objectives: 2.5x, 5x, 10x, 20x, 40x (dry), 40x (water), 40x, 63x, 100x (oil)


The Leica DMi8 is a versatile widefield microscope system suitable for fluorescence, brightfield, contrast method (phase contrast, DIC, polarized), histology color imaging and live-cell imaging (with OKO lab on-stage incubator). The system can be used to image multiple sample types including slides, petri dishes, multi-well plates, and small flasks. It is also capable of large tile stitching and can be operated as a whole slide scanning instrument.


Download Leica DMi8 user manual by clicking

Leica DMi8 operation manual

Download Leica DMi8 info sheet by clicking

Leica DMi8 info sheet

Leica DMi8 Turn On Procedure

Leica DMi8 LAS X Startup procedure

Lif2Tif Converter

Lif2Tif convert all individual images in a Leica .lif file into Tiff format. Download “Lif2Tif” by clicking link below (change file extension from .txt to .ijm before running)


Lif2Tif instruction video




PUBLICATION ACKNOWLEDGEMENT: If support from the NeuroTechnology Studio results in a research paper or other public presentation, please acknowledge this support by including the following statement in your publication(s):


“We thank the NeuroTechnology Studio at Brigham and Women’s Hospital for providing [as applicable] instrument access and consultation on data acquisition and data analysis. “


Image Gallery

Brain Tile Scan
Brain Tile Scan on Leica DMi8
Mouse Intestine
Mouse Intestine on LSM880
Mouse Intestine
Mouse Intestine Brightfield on Leica DMi8