Agilent Seahorse XFe96


System location: HBTM 7th floor 07006

To schedule a training session or pilot on the system, please contact Lai Ding (

Reservation and training requests are through the PCMS website.

The Agilent Seahorse XFe96 Analyzer measures oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a 96-well plate, interrogating key cellular functions such as mitochondrial respiration and glycolysis. XF Analyzers perform compound addition and mixing, label-free analytical detection, and automatic measurement of OCR and ECAR in real time.

There is a small incubator adjacent to the instrument, for storing cells prior to testing.


Download Seahorse XFe96 Wave manual by clicking

Seahorse XFe96 WAVE manual

Download XFe96 sample preparation manual by clicking

XFe96 Cartridge Hydration

XFe96 Cell Seeding

XFe96 Planning Material


A video of online training on Seahorse XFe96

online training by Kevin Bittman (Agilent)


PUBLICATION ACKNOWLEDGEMENT: If support from the NeuroTechnology Studio results in a research paper or other public presentation, please acknowledge this support by including the following statement in your publication(s):


“We thank the NeuroTechnology Studio at Brigham and Women’s Hospital for providing [as applicable] instrument access and consultation on data acquisition and data analysis. “