System location: HBTM 7th floor 07006D
To schedule a training session or pilot on the system, please contact Lai Ding ( lding@bwh.harvard.edu)
Reservation is through the Partners Core Management System (PCMS). Users need to register through the PCMS website (see below), then request training.
https://researchcores.partners.org/nts/about
Available Techniques: Fluorescent, Brightfield, High-Content Imaging, Live-Cell Imaging, Whole-Slide Scan
Objectives: 10x, 20x, 40x (dry)
This super-fast, sensitive, and flexible widefield cell imaging system is suitable for a broad range of high-content assays (HCA), including compound screening, predictive toxicology, protein localization and trafficking, functional studies, and stem cell analysis.
The IN Cell Analyzer 2200 incorporates intuitive and easy-to-use tools that simplify the imaging process for different sample types and enable a wide range of applications. It makes both simple and complex high-throughput, high-content assays an everyday reality.
Highlights of the system configuration:
- 5MP high-performance large field-of-view sCMOS camera.
- Seven wavelength solid-state light source covers fluorescence dyes ranging from blue (DAPI) to infrared (Cy5) spectrum.
- Environmental chamber for temperature and humidified CO2content control.
- FRET imaging module (CFP-YFP pair).
- Transmitted light imaging module.
- Fast and robust hardware autofocus + software focus.
- Deconvolution 3-D image restoration.
- GE image analysis software fleet including “IN Carta”, “Investigator” and “Developer”.
Download GE INCELL on-off manual by clicking
Download GE INCELL info sheet by clicking
PUBLICATION ACKNOWLEDGEMENT: If support from the NeuroTechnology Studio results in a research paper or other public presentation, please acknowledge this support by including the following statement in your publication(s):
“We thank the NeuroTechnology Studio at Brigham and Women’s Hospital for providing [as applicable] instrument access and consultation on data acquisition and data analysis. “