System location: HBTM 9th floor 09012C
To schedule a training session or pilot on the system, please contact Lai Ding ( firstname.lastname@example.org)
Reservation is through the Partners Core Management System (PCMS). Users need to register through the PCMS website (see below), then request training.
Available Techniques: Confocal, Super-Resolution, Live-cell Imaging, Brightfield, DIC
Objectives: 10x, 20x, 40x (dry), 40x (water), 40x, 63x (oil)
This Zeiss flagship confocal system offers high sensitivity, enhanced resolution in x, y and z, and high image-acquisition speed in one system. The patent Airyscan feature extends the system’s ability on Sensitivity, Resolution and Speed. Users achieve a 1.7× higher resolution in all spatial dimensions, 140 nm laterally and 400 nm axially. The improved sensitivity leads to better image quality and increased speed. The whole imaging process is possible with standard sample preparation and labeling protocols. The Zeiss LSM880 lets the user take full advantage of large fields of view and the highest speed of any linear scanning confocal microscope at an unsurpassed image quality.
CZI2Tif convert all individual .CZI images in a raw data folder into Tiff format. Download “CZI2Tif” by clicking the link below (change file extension from .txt to .ijm before running)
PUBLICATION ACKNOWLEDGEMENT: If support from the NeuroTechnology Studio results in a research paper or other public presentation, please acknowledge this support by including the following statement in your publication(s):
“We thank the NeuroTechnology Studio at Brigham and Women’s Hospital for providing [as applicable] instrument access and consultation on data acquisition and data analysis. “