System location: HBTM 10th floor 10006B
To schedule a training session or pilot on the system, please contact Lai Ding ( firstname.lastname@example.org)
Reservation is through the Partners Core Management System (PCMS). Users need to register through the PCMS website (see below), then request training.
Available Techniques: Confocal, Brightfield, DIC
Objectives: 10x, 20x, 40x (dry), 40x (water), 40x, 63x, 100x (oil)
This LSM710 confocal system is equipped with multiple laser lines ranging from 405nm to 633nm, and with a 32-channel Quasar spectral detector. The system uses a Zeiss AXIO Observer Z1 inverted microscope stand with transmitted (HAL), UV (HBO) illumination sources. It can collect transmitted light images (bright field and DIC) as well as conventional and confocal fluorescence images.
CZI2Tif convert all individual .CZI images in a raw data folder into Tiff format. Download “CZI2Tif” by clicking the link below (change file extension from .txt to .ijm before running)
PUBLICATION ACKNOWLEDGEMENT: If support from the NeuroTechnology Studio results in a research paper or other public presentation, please acknowledge this support by including the following statement in your publication(s):
“We thank the NeuroTechnology Studio at Brigham and Women’s Hospital for providing [as applicable] instrument access and consultation on data acquisition and data analysis. “